human α msh elisa  (Elabscience Biotechnology)

Journal: iScience

Article Title: Sebocytes contribute to melasma onset

doi: 10.1016/j.isci.2022.103871

Figure Lengend Snippet: Effect of UVA irradiation on growth factors release in SZ95 sebocytes (A) Experimental scheme of UVA irradiation of SZ95 sebocytes. (B) Phase-contrast analysis of SZ95 sebocytes after 4-UVA 5 J/cm 2 irradiations. (C) The mRNA expression levels of POMC, EDN1, SCF, and b-FGF in SZ95 sebocytes after 24 and 48h post 1-UVA and 48h post three or 4-UVA 5 J/cm 2 irradiations. Results are presented as the mean ± SD of three independent experiments and are expressed as the fold change respect to untreated control cells (∗p < 0.05, ∗∗p < 0.01). (D) Protein quantitation by ELISA of α-MSH, EDN1, SCF, and b-FGF in SZ95 sebocytes after 24 and 48h post 1-UVA and 48h post three or 4-UVA 5 J/cm 2 irradiations. Results are presented as the mean ± SD of three independent experiments and are expressed in absolute quantities (∗p < 0.05, ∗∗p < 0.01 vs Ctr). (E) The mRNA expression levels and protein quantitation by ELISA of POMC/α-MSH, EDN1, SCF, and b-FGF in SZ95 sebocytes after 72h or 1 week post 7-UVA 5 J/cm 2 irradiations. Data represent the mean ± SD of three independent experiments. For mRNA levels, results are expressed as the fold change respect to untreated control cells (∗∗p < 0.01). For ELISA assay, results are expressed in the absolute quantities (∗∗p < 0.01 vs Ctr).

Article Snippet: Human AMSH(alpha-MSH) ELISA Kit , Wuhan Fine Biotech Co. , Cat#EH0792.

Techniques: Irradiation, Expressing, Control, Protein Quantitation, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Sebocytes contribute to melasma onset

doi: 10.1016/j.isci.2022.103871

Figure Lengend Snippet: Effect of UVA irradiation on inflammatory mediators release in SZ95 sebocytes (A) The mRNA expression levels of IL-1α, IL-1β, IL-6, IL-8, and protein quantitation by ELISA of IL-6 and IL-8 in SZ95 sebocytes after 24 and 48h post 1-UVA and 48h post three or 4-UVA 5 J/cm 2 irradiations. For mRNA levels, results are expressed as the fold change respect to untreated control cells (∗p < 0.05, ∗∗p < 0.01). For ELISA assay, results are expressed in absolute quantities (∗p < 0.05, ∗∗p < 0.01 vs Ctr). (B) PGD2, PGE2, PGF2α, LTB4, and AA quantitation by HPLC-MS/MS in SZ95 sebocytes after 24 and 48h post 1-UVA and 48h post three or 4-UVA 5 J/cm 2 irradiations. Results are expressed in absolute quantities (∗p < 0.05, ∗∗p < 0.01 vs Ctr). Data represent the mean ± SD of three independent experiments.

Article Snippet: Human AMSH(alpha-MSH) ELISA Kit , Wuhan Fine Biotech Co. , Cat#EH0792.

Techniques: Irradiation, Expressing, Protein Quantitation, Enzyme-linked Immunosorbent Assay, Control, Quantitation Assay, Tandem Mass Spectroscopy

Journal: iScience

Article Title: Sebocytes contribute to melasma onset

doi: 10.1016/j.isci.2022.103871

Figure Lengend Snippet: Effect of UVA irradiation on p38MAP kinase and p53 signaling in SZ95 sebocytes (A) Western blot analysis of phospho-p38 protein expression in SZ95 sebocytes after 1-2-4h post irradiation with UVA 2-5-8 J/cm 2 β-tubulin was used as an equal loading control. Representative blots are shown. Densitometric scanning of band intensities was performed to quantify the change of protein expression (control value taken as 1-fold in each case). (B) Western blot analysis of p53 and p21 protein expression in SZ95 sebocytes after 24 and 48h post 1-UVA and 48h post three or 4-UVA 2-5-8 J/cm 2 . GAPDH was used as an equal loading control. Representative blots are shown. Densitometric scanning of band intensities was performed to quantify the change of protein expression (control value taken as 1-fold in each case). (C) The mRNA expression levels of POMC, EDN1, SCF, and b-FGF in SZ95 sebocytes after 48h of treatment with PFTα 5 μM and one or 3-UVA 5 J/cm 2 irradiations. Results are presented as the mean ± SD of three independent experiments and are expressed as the fold change respect to untreated control cells (∗p < 0.05, ∗∗p < 0.01 vs Ctr; $ p < 0.05 vs 1-UVA irradiated cells; § p < 0.05 vs 3-UVA irradiated cells). (D) Protein quantitation by ELISA of αMSH, EDN1, SCF, and b-FGF in SZ95 sebocytes after 48h of treatment with PFTα 5 μM and one or 3-UVA 5 J/cm 2 irradiations. Results are presented as the mean ± SD of three independent experiments and are expressed in absolute quantities (∗∗p < 0.01 vs Ctr; $ p < 0.05 vs 1-UVA irradiated cells; § p < 0.05 vs 3-UVA irradiated cells).

Article Snippet: Human AMSH(alpha-MSH) ELISA Kit , Wuhan Fine Biotech Co. , Cat#EH0792.

Techniques: Irradiation, Western Blot, Expressing, Control, Protein Quantitation, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Sebocytes contribute to melasma onset

doi: 10.1016/j.isci.2022.103871

Figure Lengend Snippet: Melanogenesis promotion and growth factors induction in e x vivo and in vivo systems (A) Experimental schemes and macroscopic visualization of ex vivo skin explants coculture with UVA irradiated SZ95 sebocytes. (B) Experimental schemes and macroscopic visualization of ex vivo skin explants cultured with conditioned medium from irradiated SZ95 sebocytes. (C and D) Pigmentation of ex vivo skin explants visualized by Fontana-Masson staining. Scale bars: 50 and 20 μM for low and high magnification, respectively. (E and F) Immunohistochemical analysis of SCF expression. Scale bars: 50 and 20 μM for low and high magnification, respectively. (G) Immunohistochemical analysis of SCF expression in the sebaceous glands of ex vivo skin explants stimulated with conditioned medium from control and irradiated SZ95 sebocytes. Scale bars: 50 μM (H) Western blot analysis of p53 protein expression on sebutape from lesional (L) or non-lesional (NL) melasma skin. GAPDH was used as an equal loading control. Representative blots are shown. Densitometric scanning of band intensities was performed to quantify the change of protein expression (NL control value taken as 1-fold in each case). (I) Protein quantitation by ELISA of α-MSH, EDN1, SCF, and b-FGF in sebum samples. Results are presented as the mean ± SD and are expressed in absolute quantities (∗p < 0.05 vs Ctr).

Article Snippet: Human AMSH(alpha-MSH) ELISA Kit , Wuhan Fine Biotech Co. , Cat#EH0792.

Techniques: In Vivo, Ex Vivo, Irradiation, Cell Culture, Staining, Immunohistochemical staining, Expressing, Control, Western Blot, Protein Quantitation, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Sebocytes contribute to melasma onset

doi: 10.1016/j.isci.2022.103871

Figure Lengend Snippet:

Article Snippet: Human AMSH(alpha-MSH) ELISA Kit , Wuhan Fine Biotech Co. , Cat#EH0792.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Ex Vivo, Software, Modification

Journal: Experimental Dermatology

Article Title: UV‐B‐activated B16 melanoma cells or HaCaT keratinocytes accelerate signaling pathways associated with melanogenesis via ANGPTL 2 induction, an activity antagonized by Chrysanthemum extract

doi: 10.1111/exd.13862

Figure Lengend Snippet: Angiopoietin‐like protein 2 ( ANGPTL 2) expression induces factors required for melanin synthesis in melanocytes and keratinocytes. Copy number of Angptl2 cDNA per µl after real‐time RT ‐ PCR in B16 cells with (n = 3) or without (n = 3) UV ‐B irradiation (A). Relative ANGPTL 2 protein levels in culture medium of Angptl2 ‐overexpressing ( Angptl2 ) (n = 2) vs Control (n = 2) B16 cells. Results in B are shown as means ± ranges (B). Relative melanin protein content in Angptl2 ‐overexpressing ( Angptl2 ) (n = 3) vs Control (n = 3) B16 cells (C). Relative expression of Tyr mRNA in Angptl2 ‐overexpressing (n = 3) and vs Control (n = 3) B16 cells (D). Relative expression of Mitf (E) , Trp1 (F) and Trp2 (G) mRNA s in Angptl2 ‐overexpressing (n = 3) and vs Control (n = 3) B16 cells. Copy number of Angptl2 cDNA per µl after real‐time RT ‐ PCR of HaCaT cells with (n = 3) or without (n = 3) UV ‐B irradiation (H). Relative ANGPTL 2 protein levels in culture medium of Angptl2 ‐overexpressing (n = 2) vs Control (n = 2) HaCaT cells. Results in I are means ± ranges (I). Relative α‐ MSH protein levels in culture media from Angptl2 ‐overexpressing (n = 4) and Control (n = 4) HaCaT cells (J). Relative ET ‐1 protein levels in culture media from Angptl2 ‐overexpressing (n = 4) vs Control (n = 4) HaCaT cells (K). Data from controls was set to 1. Error bars show SEM (A, C, D, E, F, G, I, J and K). ** P < 0.01, Student's t test

Article Snippet: Respective α‐MSH and ET‐1 protein levels in HaCaT cell culture medium were measured by ELISA using an α‐MSH Assay Kit (CUSABIO, Hebei, China) and an ET‐1 Assay Kit (Abcam, PLC, Cambridge, UK), according to manufacturers’ instructions.

Techniques: Expressing, Quantitative RT-PCR, Irradiation, Control

Journal: Experimental Dermatology

Article Title: UV‐B‐activated B16 melanoma cells or HaCaT keratinocytes accelerate signaling pathways associated with melanogenesis via ANGPTL 2 induction, an activity antagonized by Chrysanthemum extract

doi: 10.1111/exd.13862

Figure Lengend Snippet: CE suppresses α‐ MSH and ET ‐1 expression in keratinocytes. Relative expression of Angptl2 mRNA s in UV ‐B‐exposed HaCaT cells, with (n = 3) or without (n = 3) CE treatment (A). Relative expression of Edn1 mRNA , which encodes ET ‐1, in UV ‐B‐exposed HaCaT cells, with (n = 3) or without (n = 3) CE treatment (B). Relative expression of Pomc mRNA , which encodes α‐ MSH , in UV ‐B‐exposed HaCaT cells, with (n = 3) or without (n = 3) CE treatment. (C). Representative image showing immunoblotting for NFAT c2 protein in UV ‐B‐exposed HaCaT cells treated with or without CE (0.5 mg/mL). Hsc70 served as a loading control (D: left panel). Relative expression of NFAT c2 protein in UV ‐B‐exposed HaCaT cells, treated with or without CE (0.5 mg/mL) (n = 3, respectively) (D: right panel). Representative image of immunoblotting for NFAT c2 protein in UV ‐B‐exposed B16 cells treated with or without CE (0.5 mg/mL). Hsc70 served as a loading control (E: left panel). Relative expression of NFAT c2 protein in UV ‐B‐exposed B16 cells treated with or without CE (0.5 mg/mL) (n = 3, respectively) (E: right panel). Values from control HaCaT or B16 cells not treated with either UV ‐B or CE (0.5 mg/mL) were set to 1. Error bars show SEM (A, B, C, D and E). * P < 0.05, ** P < 0.01 by Student's t test. Schema showing melanin production promoted by ANGPTL 2 and suppressed by CE (F)

Article Snippet: Respective α‐MSH and ET‐1 protein levels in HaCaT cell culture medium were measured by ELISA using an α‐MSH Assay Kit (CUSABIO, Hebei, China) and an ET‐1 Assay Kit (Abcam, PLC, Cambridge, UK), according to manufacturers’ instructions.

Techniques: Expressing, Western Blot, Control